DNA is read in the 3 prime to 5 prime direction, and RNA is read 5 to 3.
During DNA replication, DNA polymerase synthesizes new daughter strand in 5' to 3' direction as it can add new nucleotide molecules at 3'-OH group of growing strand only. During transcription, RNA polymerase also synthesizes RNA molecules in 5' to 3' direction. Thus, the template DNA is always read by DNA polymerase or RNA polymerase in 3' to 5' direction.
During translation, a long polypeptide chain is made by reading information from mRNA molecule. The RNA molecule during protein synthesis is always read in 5' to 3' direction, from start codon to stop codon.
Thus, the correct answer to fill in first blank is 3' to 5' and in second blank is 5' to 3'.
When mRNA is made from DNA, the RNA polymerase reads the DNA in the 3’ to 5’ direction. But it too is built in the 5’ to 3’ direction.
The mRNA is read by the ribosome in the 5’ to 3’ direction.
So I would
DNA is always read in the direction 3' to 5', whereas RNA is read in the 5' to 3' direction.
I just got this question on Edginuity and got it right.