A new cream was developed to reduce the irritation caused by poison ivy. To test the effectiveness, researchers placed an ad online asking for volunteers to participate in the study. One hundred subjects replied, and they were informed that one group would receive the new cream and the other group would receive a cream with no active ingredient. All 100 subjects were exposed to poison ivy. Fifty were then assigned to the group with the new cream, and 50 were assigned to the group with the cream with no active ingredient. Which of the following is a benefit of having replication in the experiment?

Repeating the experiment will allow the researchers to compare the differences between the subjects that received the new cream both times.
Having a large number of subjects receiving the treatments will allow for a good comparison of the differences in level of irritation between the two groups.
Having a large number of subjects receiving the treatments will not allow for a good comparison of the differences in level of irritation between the two groups.
Replication is not needed in this experiment. One subject for each type of treatment is enough to determine if the reduction in level of irritation is due to the new cream.