How would evolution be influenced by the situation below. road cutting through a rain forest ?

A road cutting through a rain forest would influence evolution on reproduction aspects, since it could cause reproductive isolation. Reproductive isolation means that the species won't mate anymore, due to this physical barrier, and then they will adapt in different ways, structures or aspects. After some time being exposed to the natural selection, these individuals from both sides will have diverged so much from each other and won't have any contact with each other's genes that they will not be able to reproduce between themselves anymore, being now two different species.