I wanna be the first to give out brain if u mark me brain

Look at this painting by leonardo da vinci. this painting demonstrates da vinci's use of which of these to enhance the emotion of a painting

To make an etching the artist first coats the entire printing plate with an acid-resistant substance called a made from beeswax, asphalt, and other material. the artist then draws on the coated plate with an etching needle exposing the metal. next the plate is dipped in the plate is removed, the waxy coating removed, and the plate is cleaned, inked and printed.

Which style of polyphony uses a borrowed chant and arranges several new melodies and texts above and unrelated to the chant?