List the steps of the gram stain technique and describe the purpose of each. be sure to include a description of the color of the bacteria during each step.

The procure of gram staining is as follows:

1. Prepare the smear on a microscope slide and heat-fix it

2.Flood the smear with crystal violet on a staining tray

3.Gently and lightly rinse the smear with sterile water from a wash bottle

4.Flood the smear with iodine for a minute

5.Rinse the smear like in step 3

6.Pour 95% ethyl alcohol or acetone to decolorize for 10 seconds

7.Rinse like in step 3

8.Flood the smear with safranin (a counter-stain) for about 1 minute

9.Rinse the smear like in step 3

10.Leave the smear to air dry and observe under a microscope

Heat-fixing adheres the smear to the slide and hence makes it less amenable to being washed away during gram-staining procedure. Gram staining using crystal violet allows the peptidoglycan layer of the Gram + bacteria to pick up the dye and retain it even after decolorizing. Gram- bacteria lose the crystal violet color after decolorizing with alcohol since they lack the peptidoglycan layer. Safranin is a counter stain since it is picked by the Gram – bacteria that have lost the crystal violet dye. Therefore under a microscope and after the staining procedure, Gram+ bacteria appear violet/purple in colr while Gram – appear pink.

children tend to take more after their grandparents more than their parents. it’s the dna you received that was passed down to you somewhere in your family. maybe one of your grandparents had/has straight hair and you might have gotten it from them.

preservation because he preserves part of the land and preserves means save

explanation: