What are the disadvantages of transmission electron microscope?

A Transmission Electron Microscope is an impressive instrument with a number of advantages such as:

TEMs offer the most powerful magnification, potentially over one million times or more

TEMs have a wide-range of applications and can be utilized in a variety of different scientific, educational and industrial fields

TEMs provide information on element and compound structure

Images are high-quality and detailed

TEMs are able to yield information of surface features, shape, size and structure

They are easy to operate with proper training

Disafvantages:

Some cons of electron microscopes include:

TEMs are large and very expensive

Laborious sample preparation

Potential artifacts from sample preparation

Operation and analysis requires special training

Samples are limited to those that are electron transparent, able to tolerate the vacuum chamber and small enough to fit in the chamber

TEMs require special housing and maintenance

Images are black and white

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