Four restriction digestion reactions were performed using the plasmid containing the ligated pcr product and one or more restriction enzymes. the result of each restriction digest was then run on an agarose gel. use the plasmid map from part e to you determine which restriction enzymes were used for each reaction.

State officials are considering constructing a road through a forested wilderness area. this action will likely affect the forest ecosystem in various ways. part a: predict how the construction of a road could negatively affect plants and animals in that ecosystem. (3 points) part b: describe one way that the construction of a road could have a positive impact of the forest ecosystem. (1 point)

Glucose is broken down in different ways, both in the presence and in the absence of oxygen. using the table for reference, what major products are formed in each reaction set?

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