Select the procedure that will not saturate a thin-layer chromatography (tlc) developing chamber with developing solvent vapors.

The correct answer is adding ninhydrin to developing solvent.

Ninhydrinis used to detect (color) amino-acids, since amino acids are colourless compounds. So, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. It reacts with α- amino acids that results in purple coloured spots.  

stimulus is a change in the environment that causes an organism to react. the response is the reaction to the stimulus. some examples of stimuli for zebras are drought, to many predators, and bad feeding grounds such as dead grass or plants. zebras respond to stimuli just like all other living organisms