Which reason best explains why dead specimens must be used with transmission electron microscopes?

The specimen are placed in vacuum while examining the cells or living organisms. Also, the electron must penetrate inside of the cell and in such conditions, any living organism is not able to survive. Thus, this reason best explains the answer to why dead cells specimen must be used for Transmission electron microscopy.

The truman doctrine was an american foreign policy whose stated purpose was to counter soviet geopolitical expansion during the cold war. it was announced to congress by president harry s. truman on march 12, 1947, and further developed on july 12, 1948, when he pledged to contain threats to greece and turkey