Which of the following is not true about enzymes? they increase the \delta g of reactions. they are usually made of amino acids. they lower the activation energy of chemical reactions. each one is specific to the particular substrate, or substrates, to which it binds.

Enzymes do not increase delta G of a reaction. Delta G remains the same in an enzymatic reaction. Enzymes fasten reactions by either decreasing the activation energy or by producing binding energy and in both of these cases there is no impact on delta G so it remains the same, that is why statement 1 is incorrect. Statement 2 is correct because most of the enzymes are made up of proteins which is a polymer of amino acids. Statement 3 is also correct because enzymes lower the activation energy as already mentioned. Statement 4 is correct too. Enzymes are very specific to the kind of substrate to which they bind. Enzymes are bond specific, stereo specific & group specific of the substrate to which they bind.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

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