Which scientific studie was made by the development of the light microscope?

Actually, light microscopes date at least to 1595, when Zacharias Jansen (1580–1638) of Holland invented a compound light microscope, one that used two lenses, with the second lens further magnifying the image produced by the first. A compound light microscope gathers light from a small area (where your specimen is on the stage) and sends this light up through the objective lens. The objective lens magnifies the sample, as do the eyepieces you are looking through.

Explanation:

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