Ethanol was used as a blank to factor light absorbed by in the ethanol-chlorophyll solution.

Ethanol was used as a blank to factor the amount of light absorbed by the solvent in the ethanol-chlorophyll solution.

By using a blank, the spectrophotometer is calibrated to zero, prior to measuring the chlorophyll solution. This "subtracts" the light absorption of the ethanol so that only the chlorophyll light absorption will be measured.

lysosomes contain a variety of enzymes, enabling the cell to break down various biomolecules it engulfs, including peptides, nucleic acids, carbohydrates, and lipids (lysosomal lipase). the enzymes responsible for this hydrolysis require an acidic environment for optimal activity. hope i !