Why are biologists still debating the classification of protozoans?
a. biologists never agree on anything
b. there are estimated to be over 50,000 different species
c. protozoans can be heterotrophic or autotrophic
d. protozoans can be unicellular or multicellular

i don't know. the other person does though.

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.

tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.