Deforestation is an especially serious problem in tropical rain forests because

Seventy percent of Earth's land animals and plants live in forests, and many cannot survive the deforestation that destroys their homes. The trees of the rainforest that provide shelter for some species also provide the canopy that regulates the temperature, a necessity for many others.

Explanation

Deforestation is the permanent destruction of forests in order to make the land available for other uses. An estimated 18 million acres (7.3 million hectares) of forest, which is roughly the size of the country of Panama, are lost each year, according to the United Nations' Food and Agriculture Organization (FAO).

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.