Which of the following investigations would shed the most light on the future distribution of organisms in temperate regions that are faced with climate change?

remove, to the mineral soil, all of the organisms from an experimental plot, and monitor the colonization of the area over time in terms of both species diversity and abundance. quantify the impact of man's activities on present-day populations of threatened and endangered species to assess the rate of extirpation and extinction. compare and contrast the flora and fauna of warm/cold/dry/wet climates to shed light on how they evolved to be suited to their present-day environment. look at the climatic changes that occurred since the last ice age and how species redistributed as glaciers melted, then make predictions on future distribution in species based on past trends.