Could someone explain this further?
(this is a knockout mutation)
once you have successfully generated your mouse line, you want to perform pcr reactions using dna from the resulting animals to find those carrying the mutation. you have 3 mice: mouse 1 has two mutated copies of the gene. mouse 2 has one normal copy of the gene and one mutated copy. mouse 3 does not carry the mutation. you take your three pcr reaction products and run half of the volume in three separate wells of an agarose electrophoresis gel. draw a picture of the gel that you would expect to see (make sure to specify the sample in each lane and the size of all products in bp).
b) how do you verify the pcr product size seen on the gel?