Your supervisor asks you to set up a pure culture of a bacterial species he is researching. he tells you that this species is a fastidious, halophilic, gram-positive mesophile that does not have catalase or superoxide dismutase. what type of culture medium and environmental factors must you use to grow this organism?

This bacterial species needs to be grown in a nutrient agar of high salt (NaCl) concentration. It needs to be incubated at moderate temperature of between 20°C and 45°C. This species needs to be kept in an oxygen free environment, a sealed jar containing an oxygen- free gas mixture.

Explanation:

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.