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a lab assistant needs to mix up the following reaction to prepare dna for an experiment:
25ul of a dna sample
25ul of enzyme
50ul of reaction buffer
400ul of water
the smallest size tube that will hold the entire reaction is [0.05, 0.5, or 5] ml:
which of the following pipetting devices most likely would not be used by the lab assistant to measure the needed volumes? ? [p20, p200, p1000]?
the enzyme comes as a concentrated solution of 20 units of activity per ul. therefore, the reaction mix will have total units of the enzyme at a working dilution of units/ul.

The smallest size tube that will hold the entire reaction is 0.5ml

Explanation:

25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total

since 1000ul=1ml, then 500ul = 0.5ml

The device that mostlikely would not be used P20

Explanation:

P20 is a pipette used to measure and sample volumes of 20ul maximum.

The reaction mix will have 400 units of the enzyme.

The working dilution will be 0.8 Units/ul.

Explanation:

The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.

Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.

you did not attach the discourse, but i assume it to be the discourse on the origin and basis of inequality among men (the original titile in french is: "discours sur l'origine et les fondements de l'inégalité parmi les hommes"), also know as "second discourse". in this discourse, jean-jacques rousseau emphasizes that private property is the basis of all inequalities.

therefore, what enabled the old regime to maintain as long as it did was private property.

what in the weirdo : o