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Biology, 12.09.2019 23:30 katiebaby4109

A. you have a test tube containing 5 ml of a solution of bacteriophage, and you would like to estimate the number of bacteriophage in the tube. assuming the tube actually contains a total of 15 billion bacteriophage, design a serial dilution experiment that would allow you to estimate this number. ideally, the final plaque-containing plates you count should contain more than 10 and less than 1000 plaques. b. when you count bacteriophage by the serial dilution method as in part a, you are assuming a plating efficiency of 100%; that is, the number of plaques on the petri plate exactly represents the number of bacteriophage you mixed with the plating bacteria. is there any way to test the possibility that only a certain percentage of bacteriophage particles are able to form plaques (so that the plating efficiency would be less than 100%)? conversely, why is it fair to assume that any plaques are initiated by one rather than multiple bacteriophage particles?

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