Below are steps performed to make an e. coli bacteria that can make human insulin protein for the health care market. list these steps in their proper order.
a. use heat shock or electroporation to get the recombinant human insulin gene to go into a living e. coli cell.
b. grow the e. coli in fermentation culture, extract and sell humulin.
c. modify the human gene and give it a promoter that will be turned on in e. coli cells.
d. extract the dna from a human who does not have diabetes
e. isolate and clone (make copies of) the gene encoding the insulin protein

Are these strong bases, neutral, strong acids, weak acids or weak base household bleach (ph=12.6) fluid in a car's battery (ph of less than 1.0) milk (ph=6.7) stomach contents (ph=2.2) pure water (ph=7)

Part e - sequence of metabolic processes each of the different cellular metabolic pathways occurs in a specific order. consider aerobic cellular metabolism from the beginning to end. what is the sequential order of the metabolic processes that starts with glucose and results in the production of carbon dioxide, water, and atp? put the following in the correct sequential order. rank from earliest to latest. to rank items as equivalent, overlap them. view available hint(s) acetyl coaglycolysiscitric acid cycle pyruvate finishstartstart background image electron transport chain background image finish background image submit cellular metabolism is often expressed as an equation. this equation puts into perspective, on a general scale, the substances needed to start cellular metabolism and the products that come out of it. the equation represents the overall product yield after every step of cellular metabolism is complete (i.e., glycolysis through the electron transport system). in this part of the tutorial you task will be to put together the equation that represents cellular metabolism. you will also learn the products that result from each metabolic pathway and the role that some these products play in the production of atp.

In "the pig," what is the main effect that the piglet initially has on kibuka?