Question 15. the genome of the bacteriophage qb consists of about 4000 nucleotides of single-stranded rna. inside the e. coli host, replication of this genome requires a rna-dependent rna polymerase made up of phage and bacterial proteins. interestingly, the replicase binds to a region in the center of the rna genome, yet must start copying the 3 0 end of the rna template to produce a new strand of rna in the 5 0 to 3 0 direction. researchers hypothesized that the presence of a predicted pseudoknot in the qbgenome allowed the replicase to gain access to the 3 0 end of the rna. to test their hypothesis, they measured the replication efficiency in vitro using wild-type replicase and different versions of the qb rna containing mutations in a region key to the predicted pseudoknot formation. they specifically focused on an eight-nucleotide region from the center of the rna that was complementary to the 3 0 -terminal hairpin. the wild-type and mutant sequences and replication data are given below. wild type 5 0 -uaaagcag-3 0 guuucgucmutant a 5 0 -uaaacgag-3 0 guuucgucmutant b 5 0 -uaaagcag-3 0 guuugcucmutant c 5 0 -uaaacgag-3 0 guuugcucin vitro replication efficiency (relative to wild type)wild type: 100%mutant a: 1.6%mutant b: 0.6%mutant c: 42%a. predict why the replication efficiency so low in mutant a. b. predict why the replication efficiency is restored to almost half of wild-type levels in mutant c? c. do you think the results support or refute the hypothesis that the presence of a pseudoknot affects replication?