Astudent observes 5,000 rfus in a 200μl aliquot from a g3-500 ml culture. how many total rfus will be present in a g3-15ml sample? during rgfp purification in lab#4, assume they recover 80% of the fluorescing protein. what would be the maximum total number of rfus that they collected in the purification procedure? show your calculations for full credit.

The maximum no. of RFUs collected in the purification procedure RFUs

Explanation:

No. of RFUs in a 200 ul (0.2ml) aliquot = 5000 RFUs

So, No. of RFUs in G3-500 ml culture RFUs

                                                                RFUs  

                                                                RFUs

Similarly, No. of RFUs in a G3-15ml sample RFUs

                                                                          RFUs

                                                                          RFUs

The maximum no. of RFUs collected in the purification procedure

% RFUs

RFUs

RFUs

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