You need to make 500ml of 7h9(+) media with 1x adn and 30ug/ml of the antibiotic kanamycin. your stock solutions are 10x adn and 30mg/ml of kanamycin. the solvent is distilled water. how much of each component will you need to make the desired final solution?

ADN = 50ml

Kanamycin solution = 0.5ml

Explanation:

Final volume = 500 ml

ADN:

C1V1 = C2V2

1 * 500 = 10 * V2

V2 = ( 1 * 500 ) / 10 = 50ml

Kanamycin:

C1V1 = C2V2

30μg/ml * 500 = 30mg/ml * V2

30 * 10^-3 mg/ml * 500 = 30mg/ml *V2

V2 = (30 * 10^-3 * 500) /30

= 0.5 ml

prophase: we can observe the chromosomes to coil up and condense. they become visible. nuclear membrane and envelope disintigrate.s

metaphase: chromosomes align at the center, the metaphase plate. spindle fibers attach to centromeres of the sister chromatids. they meet at kinetochores.

anaphase: sister chromatids are pulled towards each opposing centromere, set on different poles.

telophase: chromosomes are now clustered up only on either sides of the cell. nuclear membranes reform, and a new cell wall forms along the cell plate. this will lead to cytokinesis, the process that divides the cell membrane and forms the cell wall.