The second major step of gene expression occurs in this compartment of the cell.

Ribosome

Explanation:

Expressing a gene basically means manufacturing its corresponding protein. This process has two major steps:

Ribosome

Explanation:

The second major step in gene expression is the translation. The messenger RNA (RNAm) is read according to the genetic code and translated into protein.  The translation occurs in specialized organelles called  ribosomes located in the cytoplasm. In eukaryotic cells the mRNA must be transferred from the cell nucleus to the ribosomes after transcription in order to be translated; in bacteria, mRNA is translated into protein as soon as it is transcribed. The above is because unlike eukaryotic cells, bacterias do not have a nucleus separating DNA from ribosomes, so there is no barrier that prevents  immediate translation.

Further,  immediate translation would not work in eukaryotic cells because eukaryotic RNAm must be edited and spliced prior to translation. Edition and splicing occur in  a distinct compartment in the eukaryotic nucleus prior to the beginning of translation.

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the solute can move "downhill," from regions of higher to lower concentration, relying on the specificity of the protein carrier to pass through the membrane. this process is called passive transport or facilitated diffusion, and does not require energy.