Adirect measurement of the standard free-energy change associated with the hydrolysis of atp is technically demanding becaus accurately. the value of age can be calculated indirectly, however, from the equilibrium constants of two other enzymatic reactions having less favorable equilibrium constants:

glucose 6-phosphate+ h2o → glucose+pi
(k'eq1 ' = 270)
atp+ glucose --> adp+ glucose 6-phosphate
(k'eq2 = 890)
using this information for equilibrium constants determined at 25 °c, calculate the standard free energy of hydrolysis of atp number kj/mol