5. (3pts) chromatography columns have a limited protein binding capacity. during lab #4 you should not have "overloaded" the ni+2 agarose column. yet you may have noticed that the w2 fraction fluoresced slightly. if you see a band in the western blot w2 lane, what does this data say about the physical protein structure of rgfp in that lane? give a possible mw for this band based upon the data (show your work).