Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (gfp) and the insertion of the plasmid into a bacterial cell by placing the steps in order
-ligate together the gfp gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation
-use pcr to amplify the gene for gfp
-perform a restriction digestion of the gfp gene and the plasmid
-plate the bacterial cells, and screen for positive transformants

Ameadow ecosystem includes many species of grasses and small shrubs, several herbivores, and a few carnivores. a fungus colonizes the meadow and kills most of its vegetation. what is most likely to happen to the populations of herbivores and carnivores in the ecosystem? a. the populations of herbivores and carnivores will increase because there will be less competition for chemical energy. b. the populations of herbivores and carnivores will remain stable because the fungus cannot infect these organisms. c. the populations of herbivores will decrease because of vegetation loss, but the population of carnivores will remain stable. d. the populations of herbivores and carnivores will decline because less chemical energy will be stored in the ecosystem.

The health, management and conservation of biodiversity, is a challenge facing ecosystems worldwide. the major factors that affect the health and function of the great barrier reef are climate change and pollution. which question investigates the effects of a biotic factor on the reef ecosystem?

Imagine that certain laws of physics could be ignored and you were able to travel vast distances in moments. now imagine that you traveled to an earth-like planet located light-years away that is known to support life. think about what you’ve learned in this unit and make an argument for what you think would be the dominant type of life form on this planet. consider whether a notochord is required for an organism to manipulate its environment and become a dominant creature.