Biology, 25.11.2019 22:31 solphiafischer

You decide to design an assay for flu infection detection. ha protein is only present on the surface of influenza viral particles so you choose to design an elisa assay to detect the presence of ha in human blood samples.

you will use three different antibodies for the assay. the experimental procedure is as follows:

step 1. incubate your assay plate with antibody (i) solution overnight to coat the surface of the assay plate with antibody (i). remove antibody (i) solution and wash the plate extensively.

step 2. add blood samples to the coated plate and incubate for 30 min. remove blood sample and wash the plate extensively.

step 3. add antibody (ii) solution to the plate and incubate for 30 min. remove antibody (ii) solution and wash the plate extensively.

step 4. add antibody (iii) solution to the plate and incubate for 30 min. remove antibody (iii) solution and wash the plate extensively.

step 5. add tmb substrate to the plate and monitor color development.

Answers: 2

Show answers

Another question on Biology

Biology, 22.06.2019 01:00

The allele for curly hair is incompletely dominant. if a mother is homozygous for curly hair and the father is homozygous for straight hair, what percentage of the offspring will exhibit characteristics of both parents? 25 percent 50 percent 75 percent 100 percent

Answers: 2

Answer

Biology, 22.06.2019 01:20

Transmission electron microscope are best for viewing a) microorganisms in pond water b) internal organs of a mouse c)internal structures of a cell d) surface features of a specimen

Answers: 2

Answer

Biology, 22.06.2019 15:40

What evidence could be used to convince policy makers to change a shipping lane from going through a whale breeding ground? information on the number of all whale species currently alive information on the number of all whales hit by boats in the given area information on the number of whale deaths in the world's oceans information on the number of whale offspring born every year. (a) scientists could collect and combine data on fish populations all around the world to show that their populations are declining. (b) scientists collect and combine data on fish populations all around the world to show that their populations are increasing. (c) scientists work together and use past data to show that fish populations are becoming locally extinct in some areas. (d) scientists work together and use past data to show that some fish populations are adapting to environmental changes.

Answers: 1

Answer

Biology, 22.06.2019 21:10

When you exhale, air in you lungs moves from the does anyone know the correct answer on apex

Answers: 1

Answer

You know the right answer?

You decide to design an assay for flu infection detection. ha protein is only present on the surface...

Questions