You performed a sanger sequencing reaction and obtained the following electropherogram (a computer-generated trace of the intensity of each color's fluorescence). in this figure, a = green, c = purple, g = black, t = red. the height of the peaks is unimportant. the 5' end of the sequence is at the left of the trace.

what is the sequence of the template dna used for this sequencing reaction?

a.

5' tttgctttgtgagcggataacaa 3'

b.

3' tttgctttgtgagcggataacaa 5'

c.

5' aaacgaaacactcgcctattgtt 3'

d.

5’ ttgttatccgctcacaaagcaaa 3’

e.

3' aaacgaaacactcgcctattgtt 5'

can someone with this? i can never get more than 3/5 right:

match the following terms with their descriptions below.

question selected match
used to detect close or exact complementarity to a probe sequence

c.
high stringency

used in identifying a specific mrna from a mixture

a.
northern blot

used to quantitate the initial template concentration of an unknown relative to a standard template of known concentration

b.
template dna

reliant upon dna mismatch repair

d.
site-directed mutagenesis

refers to the sequence of interest within the sample in a pcr reaction

e.
cycle threshold method (ct)