Read the following scenario to answer the following question(s).while working with cultured mouse cells, a researcher unknowingly treated the cells with a mutagen that causes the deletion or insertion of individual nucleotides in dna. subsequently, she isolated and cultured a single cell from this group. she noticed that the progeny of this cell were not producing a certain protein and that this affected their survival. the mutation would be most harmful to the cells if it resulted in ) substitution of a base pairb) deletion of a triplet near the middle of the genec) a single nucleotide deletion near the end of the coding sequenced) a single nucleotide in the middle of an introne) a single nucleotide insertion near the start of the coding sequence

americans began to notice growing differences between the northern and southern sections of the country. there were more people living in cities in the north, which made it more urban. more people lived on farms in the south, which made it more rural. while many people were farmers in the northern states, there were now other ways to make a living, such as working in mechanical cotton gins factories manufacturing cloth, tools and other goods. people in the north supported high tariffs on goods imported from europe because the extra cost encouraged people to “buy american” instead. in the southern states, planters did not like tariffs because they sold most of their production to europe and bought their tools from there as well. southerners believed the protective tariff was something that only northern factory owners keep their profits high. so the southern politicians came up with an idea. they would opt out. they would simply ignore the protective tariff. it was ‘null and void’ in south carolina. the state would not collect the taxes on goods imported into south carolina, no matter what the president said they had to do. this decision led to the nullification crisis.

the missing space is phylum