In an hfr strain, the f episome is integrated between genes aand b. when this hfr mates with an f−strain, gene bis always transferred first to the f−cell. what is the last bacterial gene that couldpossibly (at least in theory) be transferred to the f−from the host chromosome?

The answer to this will be from your lab notes/procedure. it would be best to ask another student who participated in the lab as we do not know what was done, what enzyme or if the temperature is celsius or fahrenheit. if i were to guess though i'd say the rate stopped due to enzyme denturing at high temperature