If the egfp gene is approximately 700 bp in length, why do we predict the pcr product from positive clones in lab session 6c to be approximately 1200 bp in length? is it possible for a clone to have multiple copies of egfp inserted? could two copies be inserted? could three copies be inserted? if so, what would be the result of the pcr screen? if multiple copies of the egfp gene could be inserted, would it have more, less, or the same amount of fluorescence compared to a clone with a single copy of the egfp gene present? if your dna was not cut to completion during the restriction analysis of your transformants, what would you expect your gel to look like? would you still be able to distinguish positive from negative clones? when performing dna sequencing, you only use one primer per reaction. what would happen if you mistakenly added both a forward and reverse primer to a sequencing reaction? how would your data be affected?