Optical microscopes: explain the benefits of a higher resolution

Optical Microscope - Resolution

Explanation:

Optical microscope resolution is the shortest length between any two closely located bright points on the specimen which can be viewed clearly by the viewer.

The resolution ability of an optical microscope is specified by how it distinguishes these two airy disk points which are closely placed on the specimen.

In optical microscope, with higher magnification, the image may get blurred or unsharp which can be corrected depending upon its objective. The resolution increases with increase of the numerical aperture of the entire system.

The resolution of an optical microscope is around 200 nm with a magnification power of about 1000. This resolution is less compared to that of an electron microscope .

enzyme active site and substrate specificity

enzymes bind with chemical reactants called substrates. there may be one or more substrates for each type of enzyme, depending on the particular chemical reaction. in some reactions, a single-reactant substrate is broken down into multiple products