Arrange the following events in the proper sequence for gene cloning.
1 = incorporate gene into bacterial plasmid.
2 = isolate dna from organism containing desired gene.
3 = incorporate cloned gene into bacterial cells.
4 = fragment dna with restriction enzyme.

The proper sequence for gene cloning is:

Isolate DNA from organism containing desired gene.

Fragment DNA with restriction enzyme .

Incorporate gene into bacterial plasmid .

Incorporate cloned gene into bacterial cells.

Explanation:

To start with the gene cloning process, we first must isolate the DNA from the cell and purify it.

Then, to be sure that I will obtain my desired gene and not a different DNA sequence, we must fragment the DNA with a restriction enzyme, which will cut a precise sequence, called restriction site.

Then, we must insert the gene into a vector, like a bacterial plasmid. A vector is a DNA molecule used to transport cloned sequences between the essay tube and biologic hosts.

Finally, the vector containing the cloned gene must be incorporated in our biologic hosts, in this case, a bacterial cell.

yes? sounds right, but you didn’t ask a question.

b brown mice / white sand dunes