Amicroscope technique that will not allow one to see specific parts of living specimen is

a) using stain under a light microscope

b) using a scanning electron microscope

c) passing light from a microscope through a pinhole

d) using a fluorescent substance with a specimen under a light microscope

i know that sometimes with using a stain the specimen can be killed, but im not sure if thats always the case. in advance for your