Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (gfp) and the insertion of the plasmid into a bacterial cell by placing the steps in order

-ligate together the gfp gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation

-use pcr to amplify the gene for gfp

-perform a restriction digestion of the gfp gene and the plasmid

-plate the bacterial cells, and screen for positive transformants

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid

-transform bacteria with the recombinant plasmid using electroporation

-plate the bacterial cells, and screen for positive transformants

sadness is not good

explanation:

spiders belong to a group of arachnids.

arachnids have the following traits :

they have two body parts.

they have eight legs unlike insects which have 6 legs.

they have no wings.

these traits distinguish them from insects.

the information that antonio is doubting us the statement that spiders differences are as a result of being first to evolve.

this is because the evolution of one species is independent of the other species.