You amplify and sequence the integrated construct and flanking regions in both directions. on one side, your construct is flanked by a 5000-bp region of largely random sequence. on the other side, your construct is flanked by 200 "ttaggg" repeats. based on these results, propose a hypothesis as to why your construct is not expressed. you decide to reinject the same construct into another mouse embryo in an attempt to integrate the construct elsewhere in the genome. you are happy to find that this mouse does show gfp expression in every cell.

you didn't give any options but heres what i can tell you: an inference is likely to change based on additional observations. the latter is something that you have seen or sensed using your sensory organs while the former is like a conclusion out from what you have observed.

estuaries are the partially enclosed coastal bodies of brackish water formed at the mouth of sea where river meets an ocean. it is a transition zone between river and sea water so estuaries are influenced to both marine environment such as tides, waves and to riverine such as flow of fresh water and sediments. the mixing of sea and fresh water provide high level of nutrients, making estuaries a high product habitats.

many estuaries suffers degradation from a variety of human activities like erosion from deforestation, overgrazing, overfishing, drainage and eutrophication.

so from the above statement answers will be

1. false as estuaries are formed due to both brackish and freshwater.

2.true, due to high productivity estuaries are breeding ground for many marine species.

3. direct and indirect activities of man that contribute to pollution and deposition of sediments.

bacterium is not correct

answer: dna

because all cells have genes