What are the main differences and similarites between the
radioimmunodiffusion (rid) and oucterlony plate technique?

- Similarities both are diffusion process

- wells are involved.

Radial Immunodiffusion (RID) Quantitative test technique quantitative radial immunodiffusion is based upon the reaction between an Ag, and a specific Ab during a diffusion period.

• Ag placed in a well diffuses into an agar containing the Ag (anti-IgG looking for serum IgG).

• The Ag-Ab interaction is manifested by well-defined ring of precipitation around the Ag well.

Explanation:

While Ouchterlony double diffusion (Immunodiffusion) Qualitative test It is based upon the simultaneous application of Ag and Ab in separate but adjacent wells of an agar plate.

• As the materials diffuse toward one another, ppt. lines form resulting from the Ag-Ab interactions.

• If multiple wells of Ag are positioned around an Ab well on the same plate, several patterns of reactivity may be observed.

c

explanation:

many other transport proteins, often called pumps, move materials against a concentration gradient. active transport drives molecules across a membrane from a region of lower concentration to a region of higher concentration. this process, shown in figure 5.1, uses transport proteins powered by chemi- cal energy.