1. what procedures would you follow to cause a supercompensation of muscle glycogen?

classical and Modified methods.

These methods increase the muscels glycogen to high levels,the increased muscles glycogen levels can last for about 5 days.

The classical methods involves undegoing strenuous activities for days to deplet the glycogen stored in the cells of the liver,muscles cells,and increase consumption of fatty diet while training for 3days and increase carbohydrate intake for another 3 days but not training.

Modifeid training involved 40-90 minutest raining for days while on 50 %carboyhdrate diet combined with walk out for 2 days while on 70 %Carbohydrates diet.

This should be rounded up with intake of 70 % carboyhdrate diet while resting prior the eventsm

Explanation:

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.