In an experiment with an enzyme, the 58th amino acid seems to form a covalent bond with a substrate molecule as part of the catalytic process. what would you say?

At some point the bond between the amino acid and the substrate must break.

Explanation:

Enzymes are the biocatalyst that can increase the speed of the biochemical reactions of the living organisms. All enzymes are made of proteins except ribozymes.

During the catalytic process, the enzymes can form covalent modification. This is only possible when the bonds between the substrate and amino acid is broken. This breaking and formation of bond is important so that enzyme can makes the substrate free and the steps can be repeated again.