You have isolated a decapeptide (a 10-residue peptide) called FP, which has anticancer activity. Determine the sequence of the peptide from the following information. (Note that amino acids are separated by commas when their sequence is not known.)
(a) One cycle of Edman degradation of intact FP yields 2 mol of PTH-aspartate per mole of FP.
(b) Treatment of a solution of FP with 2-mercaptoethanol followed by the addition of trypsin yields three peptides with the composition (Ala, Cys, Phe), (Arg, Asp), and (Asp, Cys, Gly, Met, Phe). The intact (Ala, Cys, Phe) peptide yields PTH-cysteine in the first cycle of Edman degradation.
(c) Treatment of 1 mol of FP with carboxypeptidase (which cleaves the C-terminal residue from peptides) yields 2 mol of phenylalanine.
(d) Treatment of the intact pentapeptide (Asp, Cys, Gly, Met, Phe) with CNBr yields two peptides with the composition (homoserine lactone, Asp) and (Cys, Gly, Phe). The (Cys, Gly, Phe) peptide yields PTH-glycine in the first cycle of Edman degradation.
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You have isolated a decapeptide (a 10-residue peptide) called FP, which has anticancer activity. Det...
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