A protein contains an N-terminal ER signal sequence followed by several negatively charged amino acids, an internal ER stop-transfer sequence, and an internal ER start-transfer sequence (in order from N-terminal to C-terminal). The signal sequence is cleaved by signal peptidase upon lateral release from sec61. How will the protein orient in the ER membrane?

A. Lumenal N-terminal, cytosolic C-terminal
B. Lumenal N-terminal, cytosolic protein loop, lumenal C-terminal
C. Cytosolic N-terminal, lumenal protein loop, cytosolic C-terminal
D. Membrane-bound N-terminal, lumenal protein loop, cytosolic protein loop, lumenal C-terminal
E. The protein will be soluble and released into the ER lumen