Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzymes X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, . but not for X. Your strategy should be to a. insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid. b. cut the plasmid with restriction enzyme X and insert the fragments cut with restriction enzyme Y into the plasmid. c. cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. d. cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments e. cut with restriction enzyme X. cut the plasmid with restriction enzyme X and then insert the gene into the plasmid.
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Biology, 22.06.2019 01:00
Plz genetic engineering can be applied to many fields including medicine and agriculture. which of the following is a medical application of genetic engineering? a. giving crop plants recombinant dna so that they would be resistant to herbicides. b. examining a persons pedigree to determine whether they can carry a gene for a genetic disease. c. analyzing a persons dna to see how closely they are related to another person. d. certain genes into bacteria so that they will produce a needed medicine.
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Biology, 22.06.2019 16:30
How do disease caused by bacteria and disease caused by viruses react to antibiotics
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Biology, 22.06.2019 19:40
Cloning an individual usually produces orangisms that (1) contain dangerous mutations (2) contain identical genes (3) are identical in appearance and behavior (4) produce enzymes different from the parent
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Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genom...
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