Biology, 21.02.2020 23:51 arturocarmena10
You will estimate the quantity of DNA present in the pUC18 DNA prep by gel band brightness. Compare your linear plasmid band in the HindIII digest lane to the λ-HindIII marker. If, for example, your linearized plasmid is half as bright as the 4, 361 bp band of the marker, then your approximate DNA quantity is 90/2 or 45 ng. You loaded 10 µl of the HindIII digest onto the gel so your original concentration of DNA in the digest is 45 ng/10 µl or 4.5 ng/µl. You put 10 µl of DNA in your digest. All together then, you have 4.5 ng/µl x 10 µl or 45 ng of DNA in the digest.
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Been sitting here trying to take this test and don't know this answer! place the events of a feedback mechanism associated with body temperature in the correct order. ·nerve cells send message from skin to the brain ·body returned to normal temperature around 98.6 degrees f ·temperatures regulation center in the brain sends out signals ·body temperature exceeds 98.6 degrees f ·sweat glands throughout the body activate to cool off skin surface it is a 1 through 5 question in biology!
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You will estimate the quantity of DNA present in the pUC18 DNA prep by gel band brightness. Compare...
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