A student observes 5,000 RFUs in a 200ul aliquot from a G3-500 ml culture. How many total RFUs will be present in a G3-15ml sample? During rGFP purification in lab#4, assume they recover 80% of the fluorescing protein. What would be the maximum total number of RFUs that they collected in the purification procedure? Show your calculations for full credit.

poor water quality affects most estuarine organisms, including commercially important fish and shellfish. the pollutants that have the greatest impact on the health of estuaries include toxic substances like chemicals and heavy metals, nutrient pollution (or eutrophication), and pathogens such as bacteria or viruses.

one would be wind another would be water density

substitution is the answer in this case