An amino acid mixture consisting of alanine, phenylalanine, and lysine is to be separated by normal phase HPLC. The stationary phase is polar and the mobile phase is a nonpolar solvent. The first amino acid to be eluted will be . The last amino acid to be eluted will be:

Phenylanine; Lysine

An amino acid mixture consisting of alanine, phenylalanine, and lysine is to be separated by normal phase HPLC. The stationary phase is polar and the mobile phase is a nonpolar solvent. The first amino acid to be eluted will be Phenylanine. The last amino acid to be eluted will be: Lysine

Explanation:

In normal phase HPLC (High performance liquid chromatography), the polar stationary phase would retain the less nonpolar amino acids (alanine and lysine) while the nonpolar mobile phase would permit the quick elution of the aromatic amino acid, phenylanine. Thus, phenylanine is first eluted.

This is then followed by the elution of the amino acid with nonpolar side chain, alanine. Thus, alanine is the second to be eluted.

Lastly, the positively charged amino acid, lysine will be released by the stationary phase. Thus, lysine is the last to be eluted

prophase: we can observe the chromosomes to coil up and condense. they become visible. nuclear membrane and envelope disintigrate.s

metaphase: chromosomes align at the center, the metaphase plate. spindle fibers attach to centromeres of the sister chromatids. they meet at kinetochores.

anaphase: sister chromatids are pulled towards each opposing centromere, set on different poles.

telophase: chromosomes are now clustered up only on either sides of the cell. nuclear membranes reform, and a new cell wall forms along the cell plate. this will lead to cytokinesis, the process that divides the cell membrane and forms the cell wall.