Two kinetics experiments have been done with 5mM enzyme. One was done an inhibitor, the other was done in the presence of 0.05M Ibuprofen, a known inhibitor. Double- reciprocal plots were made from the data. The equation of the lines of each plot are shown below (units are mWmin and mM).

Without Ibuprofen: y= 0.0116x + 0.0194
With Ibuprofen: y= 0.0203x + 0.0194

a. Use the data above to fill in the table below (show your work in the space on the right):

Actual Apparent
KM
Vmax

b. Calculate the Kcat of the enzyme.

Defend or refute the argument that the government has too large a presence in the agriculture industry.

Rease is an enzyme used by plants to break down urea (a nitrogen-containing compound) into carbon dioxide and ammonia. urease urea > > > carbon dioxide and ammonia ammonia is broken down by plants into a nitrogen source plants need to grow. thus, plants could not use urea as a nitrogen source unless it was first converted to ammonia. in soybean plants there are two different kinds of urease, one produced in the seeds and the other produced in the leaves of the plant. three types of soybean plants were used in a set of experiments: normal soybeans and two mutant strains, one lacking the urease in the seeds only (strain 1) and one lacking urease in the leaves only (strain 2). experiment 1 separate areas in a field were planted with normal, strain 1, and strain 2 soybeans. all types of soybeans appeared to grow, flower, and produce seeds equally well. there were no externally detectable differences among the strains. experiment 2 small pieces of plant leaves of equal weight were obtained from each type of soybean plant and separately placed on media in culture dishes. tissue growing in this way will become an unorganized clump of cells referred to as callus. to provide a controlled nitrogen source, half the tissue samples of each type were placed on media containing urea, and the other half of the samples were placed on media containing ammonia. after 30 days, the weight gain for each of the callus samples was determined. results are shown in the table below.

2. which is the last step in the scientific method?