Biology, 02.03.2020 21:51 liljumpmanj
EML is a protein that is produced at low levels in healthy cells, but is highly expressed in cancer cells. You know that EML expression correlates with HER2 activity in cancer
cells, but you do not know if EML causes HER2 activation or if HER2 activation induces EML expression.
a. What method would you use to quantify expression of EML (Hint: should involve SYBR green)?
b. After using CRISPR/Cas9 to knock out the HER2 gene in a healthy cell line, you transform this CRISPR-edited HER2 -/- cell line into cancer cells. When comparing the expression of EML in the HER2 -/- vs the wild type cancer (assume the HER2 gene is intact) cell lines, you find that the Ct value of EML is significantly higher in the HER2 -/- cells. What conclusion about the relationship between HER2 and EML does this experiment suggest?
Answers: 3
Biology, 22.06.2019 02:30
Actinobacteria sp. are fermenting organisms (which do you use oxygen to breathe) referred to as chemoorganohetereotrophs this means they break down organic material and convert it to inorganic material. which part of the carbon cycle does this describe
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Biology, 22.06.2019 13:50
Interactions between organisms and their environment impact the organism’s overall population. the jaguar panthera onca is the largest cat in north america. it is found in areas across the southwest, including arizona, new mexico, and texas. it is a carnivore that has powerful jaws and sharp teeth and preys on fish, turtles, tapirs, and many smaller mammals. which shows the relationship between the jaguar and turtles?
Answers: 3
EML is a protein that is produced at low levels in healthy cells, but is highly expressed in cancer...
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