Bacteria are grown in N15 over time, then switched to growth in N14 medium. After three divisions (60 min) what is the percentage of bacterial cells that have N15 labeled DNA? (A) 100% (B) 50% (C) 25% (D) 10% (E) 5%

C. 25%

Explanation:

A bacterial cell gives rise to two daughter cells by one division. If a single bacterial cell enters into cell division, it will form a total of 2^3= 8 cells after three rounds of cell division. The two DNA strands of the parent DNA duplex having N15 will be present in two bacterial cells out of the total 8 cells. This would occur since the process of DNA replication forms a new DNA duplex with one parental strand and one new strand. So, after three round of cell division 25% cells will have DNA with N15 (2/8 x 100= 25%)

the calvin cycle has three stages. in stage 1, the enzyme rubisco incorporates carbon dioxide into an organic molecule. in stage 2, the organic molecule is reduced. in stage 3, rubp, the molecule that starts the cycle, is regenerated so that the cycle can continue

this protocol provides a detailed procedure for nucleoprotein extraction from cultured cells and tissues in an efficient manner.