Affinity chromatography is a method that can be used to purify cell-surface receptors, while they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads. This method of affinity chromatography would be expected to collect which of the following? A. Molecules of the hormone. B. Molecules of purified receptor. C. G protein. D. Assorted membrane proteins. E. Hormone-receptor complexes.

Affinity chromatography is a method used to separate molecules with high affinity. This technique enables the purification of specific molecules ranging form nucleic acids, enzymes, antigens, antibodies, etc. In the example, the ligand will produce the receptor to be displaced from the beads and then eluted out.

stimulus is a change in the environment that causes an organism to react. the response is the reaction to the stimulus. some examples of stimuli for zebras are drought, to many predators, and bad feeding grounds such as dead grass or plants. zebras respond to stimuli just like all other living organisms

population. population is the number of people or animals in a particular place. an example of population is over eight million people living in new york city.

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